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KMID : 0380020070220040197
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 4 p.197 ~ p.200
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Octimization of Conditions of Filtration and Concentration of Methanol Extract for Recovery of Paclitaxel from Plant Cell Culture
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Kim Jin-Hyun
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Abstract
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This study examined the conditions of filtration and concentration of methanol extract from biomass. Filtration efficiency was improved by adding diatomaceous earth as a filter aid. The optimal amount of diatomaceous earth was 6% (w/w) to reduce the filtration time. The filtration time was reduced by 4.2% in first extraction, 30.0% in second extraction, 22.8% in third extraction, and 19.0% in fourth extraction, respectively. The optimal temperature of water bath was below 50 for preventing paclitaxel degradation during concentration of methanol extract using a rotary evaporator. The temperature of concentrated solution in rotary evaporator was relatively low compared to bath temperature because of latent heat of evaporation. The stopping point of concentration in rotary evaporator for the following step was at a specific gravity of 0.96 of the concentrated solution in terms of the purity and yield of paclitaxel. This information is very useful for mass extraction of biomass for the recovery of paclitaxel from plant cell culture.
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KEYWORD
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Paclitaxel, extraction, filtration, diatomaceous earth, concentration
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